منابع مشابه
Cleavage of phycocyanobilin from C-phycocyanin.
The chromophore of C-phycocyanin, phycocyanobilin, was cleaved from the protein with methanol, concentrated hydrochloric acid, or subtilisin BPN’. The pigments obtained were converted to their dimethyl esters and purified by preparative high pressure liquid chromatography and examined for purity by analytical high pressure liquid chromatography on silica gel. They were characterized by proton t...
متن کاملCharacterization of R-phycocyanin. Chromophore content of R-phycocyanin and C-phycoerythrin.
R-phycocyanin was purified from two independent isolates of the unicellular red alga Porphyridium cruentum. At pH 7.0 the protein sediments as a single component with s 20,w of 5.98 S (at 2 mg/ml, gamma/2=0.02). Over a protein concentration range of 0.2 to 0.5 mg/ml (gamma/2=0.16), sedimentation equilibrium gave a molecular weight of 103,000 +/- 6,000 with no evidence of heterogeneity. In commo...
متن کاملC-phycocyanin incorporated into reverse micelles: a fluorescence study
The solubilization of C-PC into aerosol-OT(AOT, sodium bis(2-ethylhexyl)sulphosuccinate)/water/i-octane reverse micelles has been investigated by fluorescence spectroscopy by following its behaviour as a function of the water-to-surfactant molar ratio, w0. The maximum wavelength emission decreased steadily with an increase in water content, together with a concomitant increase in steady-state a...
متن کاملMedical Application of Spirulina platensis Derived C-Phycocyanin
Along with the development of marine biological pharmaceutical research, high-effective and low-toxic drugs and functional foods isolated from marine organisms have become a new field of pharmacy and bromatology. The pharmacological actions, such as anti-inflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of C-phycocyanin (C-PC) from Spirulina platensis...
متن کاملDissociating effect of chromophore modifications C-phycocyanin heterohexamers on
The bilin chromophores of the a or /3 subunit of C-phycocyanin (PC) from Mastigocladus laminosus were modified, and subsequently recombined with the respective complementary unmodified chromophores. The modifications consisted of photobleaching (350 nm) or reversible reduction of the verdinto rubin-type chromophore(s). Recombination led to heterodimers (a& but the heterohexameric aggregation st...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.4.525